55 US AI Startups Securing Over $100M in Funding in 2025
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Overview of the AI Industry’s Funding Landscape
As we stepped into 2025, the AI industry exhibited remarkable momentum. In 2024 alone, a total of 49 startups successfully secured funding rounds of $100 million or more. Notably, three companies achieved two “mega-rounds” or more, while an impressive seven firms raised rounds of $1 billion or higher.
Continued Growth in 2025
The trend continued unabated into 2025. Although the number of companies raising rounds exceeding $1 billion decreased, four companies did raise funding over that threshold, including Anthropic, which uniquely completed two such rounds. In total, eight companies in 2025 successfully navigated multiple funding rounds, a significant increase from the previous year.
What does 2026 hold? Early indications are promising. For instance, in the opening weeks of 2026, Elon Musk’s xAI announced a colossal $20 billion Series E round, and Sam Altman’s Merge Labs received a $250 million seed round, led by OpenAI. The early momentum of 2026 suggests a continuation of the robust growth seen in the prior years.
Notable AI Funding Achievements in 2025
December Highlights
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Mythic (Austin, TX): This company, focused on power-efficient AI computing, raised a $125 million venture round on December 17. The round, led by DCVC, included investors like SoftBank and NEA.
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Chai Discovery: Announced a $130 million Series B round on December 15, valuing the biotech-focused AI model company at $1.2 billion. Co-led by Oak HC/FT and General Catalyst, this funding marks a significant step forward for Chai.
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Fal: The generative media platform announced its third funding round of 2025, a $140 million Series D on December 9, led by Sequoia, reaching a valuation of over $4.5 billion.
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Unconventional AI: On December 8, this startup made waves with a $475 million seed round, co-led by Lightspeed Venture Partners and Andreessen Horowitz, achieving a valuation close to $4.5 billion.
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7AI (Boston, MA): Specializing in cybersecurity AI agents, they secured a $130 million Series A round on December 4, with participation from notable investors like Greylock and Spark Capital.
November Milestones
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Genspark: The all-in-one AI workspace platform raised $275 million in a Series B round on November 20, achieving a $1.25 billion valuation.
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Luma AI: This startup, focused on photo and video creation, raised $900 million in a Series C round, placing its valuation at $4 billion, led by Humain.
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Anysphere: The creator of the vibe-coding platform Cursor raised an astonishing $2.3 billion, achieving a valuation of $29.3 billion on November 13.
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Parallel: This web infrastructure company raised $100 million in a Series A round on November 12, co-led by Index Ventures and Kleiner Perkins.
October Achievements
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Fireworks AI: Announced a $250 million Series C round on October 28, valuing the company at $4 billion.
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Uniphore: The enterprise AI firm secured a $260 million Series F round on October 22, bringing its total valuation to $2.5 billion.
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Sesame: This voice AI company raised $250 million in a Series B round co-led by Sequoia on October 21.
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OpenEvidence: The medical AI chatbot platform raised $200 million in its second funding round, achieving a $6 billion valuation.
Key Trends and Takeaways for 2026
The momentum from 2025 into the early weeks of 2026 indicates that the AI sector shows no signs of slowing down. The significant funds raised by companies such as xAI and Merge Labs are encouraging indicators of further growth. Investors remain eager to back innovative AI solutions, ensuring that the landscape is not only vibrant but also full of possibilities for new ventures.
With increased interest across sectors such as healthcare, security, and media, the coming year will likely spotlight transformative startups that continue to push the boundaries of AI technology.
As we monitor the developments throughout 2026, it will be interesting to observe how these funding trends evolve and what new innovations emerge from this dynamic industry.
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